Data and code for: Sequence specificity in DNA binding is mainly governed by association

Sequence-specific binding of proteins to DNA is essential for accessing genetic information. Here, we derive a simple equation for target-site recognition, which uncovers a previously unrecognized coupling between the macroscopic association and dissociation rates of the searching protein. Importantly, this relationship makes it possible to recover the relevant microscopic rates from experimentally determined macroscopic ones. This post contains kinetic data for the lac repressor and code to analyse and interpret the data in light of this theory. File list:- data_pbm.zipOpens in a new tab - Data-set for output generated using a protein binding microarray. - data_tirf.zipOpens in a new tab - Data-set for output generated using TIRF based single molecule localisation microscopy. - data_cas9.zipOpens in a new tab - Data-set for cas9 binding collected from literature. - data_spr.zipOpens in a new tab - Data-set for output generated using surface plasmon resonance based methods. - figure_scripts.zipOpens in a new tab - Set of scripts used to generate all figures. See README file inside for details.