Immunofluorescence data of 700 host cell proteins in Sars-CoV2 infected vs non-infected VeroE6 cells
https://doi.org/10.17044/SCILIFELAB.14315777
The data set contains ≈ 75,000 confocal images of mixed Vero E6 cell populations (SARS-CoV2 infected vs non-infected). Cells had been infected with the virus for 1 h and fixed 24 hour post infection in 96 well plates. Using immunofluorescence (4 colours) cells were stained for the SARS-CoV2 virus (red), the endoplasmic reticulum (yellow) and nucleus (blue) – same for all images in the data set and used as reference markers. On top of this, 700 different target proteins were stained – one per well (green). Images were acquired with an Opera Phoenix microscope at 63X, using 9 FoV and 3 z-planes per protein target. IN the original study, intensity differences and spatial re-distribution of the target proteins were evaluated in infected vs the non-infected cells. The image resource was generated and described in detail in this research article: (to be added).
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Opens in a new tabhttps://doi.org/10.17044/SCILIFELAB.14315777
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Creator/Principal investigator(s):
- Jayasankar Mohanakrishnan Kaimal
- Ulrika Axelsson
- Trang H. Le
- Hao Xu
- Hanna Axelsson
- Anna Bäckström
- Duncan Njenda
- Elisabeth Moussaud-Lamodière
- Wei Ouyang - Science for Life Laboratory
- Carolina Oses Sepulveda
- Brinton Seashore-Ludlow
- Caroline Vernersson
- Ali Mirazimi
- Päivi Östling
- Emma Lundberg
- Charlotte Stadler
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